The changing patterns of antimicrobial resistance and shortage of novel classes of antibiotics have contributed to a demand for new antibacterial agents. coli remains a public health concern and is responsible for severe infections in humans. In addition, antibiotic resistance in foodborne E. The worldwide increase in the rate of resistance to carbapenem and colistin among members of the family Enterobacteriaceae, especially in developing countries, is a serious threat to their therapeutic use. STEC can be acquired by ingestion of contaminated food or water, or through contact with animals or their environment. It can also cause food-borne infections, such as those caused by enterohemorrhagic E. It can cause hospital- and community-acquired infections, resulting in diarrhea, meningitis, urinary tract infections (UTIs), bacteremia, pneumonia, surgical-site infections, and sepsis. coli is used as a contamination indicator for water, food, and agricultural products. It belongs to the family Enterobacteriaceae and as a pathogen causes serious urinary and gastrointestinal infections in humans. Annotation of the predicted genes revealed gene products belonging to several functional groups, including regulatory proteins, DNA packaging and phage structural proteins, host lysis proteins, and proteins involved in DNA/RNA metabolism and replication.Įscherichia coli is a Gram-negative, non-endospore-forming bacterium that is motile by means of peritrichous flagella. Comparison of predicted and experimental restriction digestion patterns allowed rough mapping of the physical ends of the phage genome, which was confirmed using the PhageTerm tool. The phage genome is 45,805 bp in length with a GC content of 50.3% and contains 76 predicted genes. The host range of the phage was found to be narrow, as it infected only two out of 137 clinical E. The diameter of the icosahedral head of fEg-Eco19 is 68 ± 2 nm, and the non-contractile tail length and diameter are 118 ± 0.2 and 13 ± 0.6 nm, respectively. Electron microscopy showed that the isolated phage is tailed and therefore belongs to the order Caudovirales, and morphologically, it resembles siphoviruses. Phage fEg-Eco19 formed clear, sharp-edged, round plaques. coli strain, was isolated from an Egyptian sewage sample. Phage fEg-Eco19, which is specific for a clinical E. The present study describes the isolation and characterization of a bacteriophage effective against the important clinical pathogen Escherichia coli, which shows increasing accumulation of antibiotic resistance. This, in turn, is important for the exploitation of phages for therapeutic purposes, as the use of uncharacterized phages may lead to treatment failure. Characterization of bacteriophages facilitates better understanding of their biology, host specificity, genomic diversity, and adaptation to their bacterial hosts.
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They are perfect for active families and families with young children. They have a lot of energy and do silly things all the time. They like to please their owners, so doing tasks and being rewarded for them make the dogs happy. Most boxers do very well with training and enjoy it. They are happy to cuddle up on the couch with their owners and enjoy being petted and hugged. Affectionateīoxers love affection and attention. Boxers are gentle and tolerate a lot of children. Great With Childrenīoxers are very social, and they do well with children. They bond with their owners quickly and become protective over them and other pets. Protective Natureīoxers are very protective by nature and were bred as guard dogs. Many people consider boxers the best dogs, and here’s why. If you are considering getting a boxer, read on to learn more about the breed. They are ideal for families, but they also have some issues that may not make them suitable for all households. They are also very protective and do well with training. Understanding the needs of a boxer and providing proper care will ensure your dog fits in with your family and is happy and healthy.Ĭheck out for more: Can Boxers Be Aggressive? and Boxer Ear Cropping – What To Understand Before You Decide Boxer Pros And ConsĪre Boxers good dogs? Boxers are good dogs that are known for being gentle and loyal. But like every other dog the Boxer has pros And cons. Boxers are fun-loving dogs that make great pets. Ground pork should always be cooked to 160 degrees F. 4 center-cut boneless loin pork chops, 4 ounces each, all fat removed 3 ounces fresh oyster or shiitake mushrooms cup canned fat-free, low-sodium chicken broth In a bowl, place the dried mushrooms and cover. Meats to avoid include: prime cuts of beef, such as ribs. Diabetic Recipe: Pork Chops With Wild Mushrooms - Recipes For Diabetics 1/8 ounce dried wild mushrooms, such as morels, mixed Italian etc. Chicken Chili with Sweet PotatoesTons of spice, corn and bell pepper give this healthy one-pot chicken chili recipe Southwestern flair. High fat meats contain 8 g of fat and 100 calories per 1-oz serving. (well), with a 3-minute rest time after leaving the grill. People with diabetes should avoid high fat and processed meats. Place on a baking sheet coated with cooking spray lightly spritz chops with cooking spray. Dip pork chops in milk, then coat with crumb mixture. High – Red glow visible through ash coatingīecause pork cuts will cook differently depending on their size and thickness, it is important to monitor pork temperature with a digital thermometer when grilling.Įnjoy the best eating experience when cooking cuts such as pork chops and pork tenderloin between 145 degrees F. In another shallow bowl, toss bread crumbs with cheese and seasonings. Medium – Coals covered with light-gray ash Low – Ash coat is thick, red glow less visible Set aside to cool then place garlic and broth mixture in blender. To check cooking temperature when using charcoal: Simmer 25 to 30 minutes or until garlic mashes easily with fork. Baste during the last few minutes of cooking. Remove the pork chops from the marinade, and add the excess marinade to a small saucepan. Let the pork chops marinate for 6 to 8 hours or overnight in the refrigerator. Sugar-based sauces (many commercial barbeque sauces) tend to burn if applied too early. Add the rest of the ingredients to a large bowl or zip-top bag. Add the remaining 2 tsp olive oil to a large sauté pan over medium-high heat. Hit the saute button on your pressure cooker (FYI, here are what all your Instant Pot buttons mean ). Steam the broccoli for 5 minutes until tender. Add the pork chops and marinate them in refrigerator for 20 minutes or longer. Use spatulas or tongs for turning.įrequently flipping pork on a cooler area of the grill is better than accidentally burning it on an area that is too hot. In a medium bowl, combine the soy sauce, vinegar, water, 1 tablespoon olive oil, crushed red pepper, and garlic. To prevent pork from sticking to the grate, scrub grates clean and coat with vegetable oil or a nonstick vegetable oil spray prior to using.ĭo not use sharp utensils that may pierce the pork when flipping, as piercing allows flavor-filled juices to escape. eBook formats supportedįor the Libra 2, its EPUB, EPUB3, FlePub, PDF, MOBI, JPEG, GIF, PNG, BMP, TIFF, TXT, HTML, RTF, CBZ, and CBR. Again, that may not be a dealbreaker for the Paperwhite considering that the latter is designed to allow reading in portrait mode. However, while the Libra 2 features an automatic rotation of page orientation, it’s missing on the Paperwhite. So, it’s largely a matter of personal choice. It’s present on the Libra 2 but not on the Paperwhite and shouldn’t be a big deal unless you love physical buttons. This way, both the eReaders are on even ground on this front. That means the ability to survive submergence at a max depth of 2 meters for up to 60 mins. You can indulge in some serious pool-side reading with either of the devices given that both come with IPX8 certification. This makes the new Kindle best suited for the long haul. Kobo isn’t revealing the numbers here yet but should easily last around a month and a half comfortably under normal usage. This means a significant advantage for the Libra 2 here.Īmazon has gone to town trumpeting how its new Paperwhite 5 series is even more frugal on battery power, allowing for around 10 weeks of usage on a single charge. If you want a bigger device, you will have to opt for the Paperwhite Signature Edition which too has 32 gigs of storage to offer but at a $10 premium. It’s 32 GB of the Libra 2 while the base Paperwhite has a puny 8 GB of storage to offer. In all, the Kindle seems to be on top here, albeit by a slim margin. The Libra H20 weighed 192 grams while the erstwhile Paperwhite weighed 182 grams. Interestingly, both the new-gen Kobo and Kindle devices are a bit heavier than their respective predecessors. In comparison, the latest Kindle has a width of 8.1 mm while tipping the scales at 205 grams for the base model and 208 grams for the Signature Edition. The Libra is also the thicker and heavier of the two, it being 9 mm in thickness and 215 grams in weight. For those who’d prefer numbers, it’s 144.6 x 161.6 mm for the Libra 2 while the Paperwhite 5 measures 124.6 x 174.2 mm. The same is designed to serve as a nice place to hold the device while reading thereby ruling out chances of an accidental touch action on the display.įurther, the Paperwhite 5 has a portrait orientation while the Libra 2 has more of a squarish stance. That means fairly slim bezels on all sides but a super-thick edge along the right. The Libra 2 however is continuing with the same basic form factor of its predecessor, the Libra H20. The Paperwhite 5 sports a whole new design that is accentuated by slimmer bezels along all sides save for the bottom. So, the Kobo seems to have a slight edge here compared to the Kindle. For the record, the Paperwhite has a smaller reading area, 6.8-inch compared to the 7-inch display of the Libra 2. For the Paperwhite 5, the resolution stands at 1430 x 1080 pixels which too makes for a pixel density of 300 PPI. That way, the Libra 2 display has a resolution of 1680 x 1264, which when spread over its 7-inch display allows for a pixel density of 300 PPI. For that, the Libra 2 features an E Ink Carta 1200 display while the Paperwhite 5 series makes do with the E Ink Carta HD. Now, both the Libra 2 and the Paperwhite 5 come across as handy devices that have been designed for the primary purpose of reading digital content. Here we are going to discuss how the new Kobo Libra 2 fares against the Kindle Paperwhite 5 base model as well as the Signature Edition. Both are aiming at the same market segment, which would make it extremely interesting to find out how one stacks up against the other. Close on the heels of Amazon launching its new Kindle Paperwhite 5 series has been Kobo that introduced its new generation Kobo Libra 2 (and Sage) eReader devices. There has suddenly been a spurt in activity in the eReader segment. Interested in adding a few old-school love songs to your must-play list? Ahead, here are a handful of tracks that Daniels says will resonate with people across every generation. More specifically, the melodies and arrangements of old-school love songs have been sampled and incorporated into new tracks across every genre, from pop and R&B to rock and hip-hop. Though this genre of music only exists when you look back in time, if you pay attention to the type of music that’s popular today, it’s evident that these songs have served as the inspiration for many modern artists. Historical and Cultural Context of Old-School Love Songs According to Daniels, artists like The Temptations, The Righteous Brothers, Earth Wind & Fire, Hall & Oates, Elvis Presley, and Michael Jackson “will forever remain evergreen,” and will always be a hit at any wedding. With that in mind, however, some artists are universally accepted as “old school,” regardless of the decade you were born in and where you grew up. “A throwback song to me at the age of 42 is completely different than to a person who in their 60s or 70s, or someone who’s in their 20s!” Simply put, as people get older, the idea of what’s considered “old school” can change. “An old-school love song can be defined by anything that was popular when you were growing up,” explains Daniels. How to Choose Music Based on Your Wedding Style An Introduction to Old-School Love Songs These tracks are also a great choice for the father-child dance or mother-child dance, especially if your parents refer to old-school love songs as their favorite type of music.Īhead, we put together a list of the best old-school love songs that are sure to create unforgettable memories at your wedding. Read on for more. However, songs from what I consider to be 'back in the day' definitely contain a sense of innocence, sweetness, romance, and purity when it came to singing about love.”įrom "My Girl" by The Temptations to "You Are the Sunshine of My Life" by Stevie Wonder and "What a Wonderful World" by Louis Armstrong, there's an array of tunes to choose from that will pull at the heartstrings of everyone at your celebration. “Today's music doesn't leave much to the imagination. “These songs have stood the test of time because of the purity,” says Keenan Daniels (professionally known as "The Million Dolla Man"), a DJ based in Tennessee. In the context of the song, Cant Help Falling in Love describes the powerful and. That's because this style of music is absolutely timeless, appeals to multiple generations, and has the ability to evoke feelings of nostalgia with its heartfelt lyrics and captivating medleys. The songs lyrics convey a deep and heartfelt sense of love and affection. So, I'm crying.No matter your taste in music, there's something about an old-school love song that will instantly get you in your feelings. <3 Comment by this is amazing! Comment by ❦᯽□□□ □□□ □□□□ □□□᯽❦īruh I had this song in my head all day Comment by ♡︎supernatural♡︎ This makes me wanna cry brb Comment by bubblesfarttart (You'll Never Walk Alone), a children's album (With a Smile and a Song), and. My wedding song Comment by bubblesfarttart Love (on the Hollywood double) even sounds as if it could be Riddle himself. I DONT LISTEN TO THESE TYPA SONGS BUT OMG THIS IS SO BEAUTIFUL! Like im mkre hiphop and shkt but dam this might change everhthkng Comment by MANAR Somebody to love Comment by Joseph Holtzapple Verse 1 Wise men say 'Only fools rush in' But I cant help Falling in love with you Verse 2 Shall I stay Would it be a sin If I cant help Falling in love with you Chorus Like a. Elvis Presley : Can't help falling in love And now he will get behind the wheel of a Toyota for 23XI. Kasey Kahne also accomplished the journeyman feat.īusch drove Chevrolets for Ganassi and Phoenix Racing. Many traditionalists cried foul, preferring to see numbers remain in their usual spot.īusch will become just the second to drive for each of NASCAR's last four manufacturers: Chevrolet, Dodge, Ford and Toyota. NASCAR first allowed teams to shift numbers for the All-Star Race at Bristol Motor Speedway. It was a clear indication that NASCAR plans to allow numbers to move on the Next Generation car to allow sponsors more prominent ad space on racecars. Instead of being in the center of the door, it was considerably closer to the front wheels. 45 on the side of the car was moved forward. When 23XI introduced its second car Friday, the video raised eyebrows because the No. So all that kind of leapfrogged up ahead of walking away from full-time NASCAR driving.” “But this is another box that I didn't know to exist and that is building a new team from scratch, working with Toyota and having a strong relationship with Monster Energy to go to a new program. “For me, I've completed quite a bit of my checklist in NASCAR," Busch said. I think Kurt instantly jumps us up to that level.”īusch welcomed the move and said the offer allowed him to put off retirement thoughts. You’ve got to figure out what it takes to win first. Our focus is going to be solely on these two guys and how can we get them up front, in the top five, every single week contending for race wins. However, for us, it’s going to be how can we get our two cars running up front before we think about doing anything further. “It’s always been a vision of ours to expand," Hamlin said. The next step, though, is making both cars competitive. “Kyle was excited to see it used," Hamlin said.Ģ3XI Racing made it clear all year that it wanted to expand to two cars and eventually more. 23XI reached out to Kyle Petty for his approval to get the car number back on track. It was piloted by both Kyle Petty and his son, Adam, who was killed at age 19 in a 2000 crash. 45 has long been associated with the Petty family. "With it being 23, then 45, that kind of made sense.” “It was number that meant something to (Jordan)," Hamlin said. 23, which is most associated with Jordan’s career. 45 when he returned to the NBA in 1995 following his first retirement. 1 Chevrolet at Chip Ganassi Racing, will move with Busch to 23XI next year. Monster Energy, which sponsors Busch's No. It was an easy startup because Wallace brings millions in sponsorship. Hamlin, a three-time Daytona 500 winner, partnered with Jordan this year to build a team around Bubba Wallace, NASCAR's only Black driver. “This has exposed hypocrisy at an untold level, this shipping across the country of illegal immigrants to these liberal jurisdictions,” she said. “But to have an illegal migrant who just crossed the southern border standing in front of the vice president’s home, who just said the border was secure, as he says. We did not come legally.’ So, this is the making of SNL skits, except it’s not funny at all. Former White House Press Secretary Kayleigh McEnany is being slammed on social media after mocking President Joe Biden for saying 'everyone knows I like kids better than people' during. KAYLEIGH MCENANY MOCKS BIDEN 'LOVING' KIDS & JAKE TAPPER HAS MELT DOWN - The Home of Conservative & Pro-Trump news, forums, videos & our popular Patriots Page. Everyone believes the border is open because we enter. Trump-hating comedian Kathy Griffin claimed White House Press Secretary Kayleigh McEnany would be reduced to doing sexual favors for Rudy Giuliani, if President Trump loses the election. The illegal immigrant says ‘it’s open, not closed. He asks an illegal immigrant, Hey, you know, is the border secure? The vice president right there over the fence in the Naval Observatory says it’s secure. Griff Jenkins brilliantly goes in front of the vice president’s residence at the Naval Observatory. “What an interesting piece of footage came out. “You asked the million-dollar question, why is it evil and inhumane for Governor DeSantis to send migrants to Martha’s Vineyard, but not evil and humane when Biden for two years sends secret flights of migrants around the nation? There’s no difference there. The core is also filled with fire-proof material. (The mould is shown here with a solid cast in plaster) Step 3: From this rubber mould a hollow wax or paraffin cast is made Step 4: The hollow paraffin apple is covered with a final, fire-proof mould, in this case clay-based, an open view. Step 1: A model of an apple in wax Step 2: From the model a rubber mould is made. Both component parts are cleaned up with a fettling knife and damp sponge.Process by which a duplicate metal sculpture is cast from an original sculpture The mold intact for a further hour or so for the form to firm up for easier handling.īoth ceramic molds are then disassembled and the jug form and handle carefully removed. When the mold is well drained and the sheen has gone from the wet casting slip (typically about 20 minutes), remove only the reservoir portion from the jug mold, trim the excess clay from the top and clean it up with a damp sponge. Tip: To achieve even thickness between multiple casts, it is a good idea to time the first casting and use this as a guideline. Placing it at an angle avoids stalactites of clay forming on the bottom of the piece. Once the slip is the desired thickness (check by blowing on the edge of the mold where the slip and plaster meet), pour the extra casting slip back into the container and leave the mold inverted at anĪngle to drain into a bucket. The handle mold is also filled with casting slip. As the porous mold absorbs the water from the clay, the excess is drawn from the reservoir. Mold to halfway up the reservoir wall using commercial earthenware casting slip. If you design your mold to include a reservoir, which makes it easier to maintain an even rim thickness, fill the To prevent leaks when pouring the casting slip, secure the parts firmly together with strong bands cut from rubber inner tubes (figure 3). The mold for the body of the jug (figure 2) is made in four parts – the two sides, the base, and With a little modification to the original jug design, I made new plaster molds, one for the handle (figure 1) and the other for the body of the form. The ceramicįorm shown here is a slip-cast jug that I designed as part of a college tableware design project. This is why I use ceramic molds to make my forms. – Jennifer Poellot Harnetty, editorĬeramic decals can be applied to any glazed object, but it makes life easier if the ceramic form has smooth surfaces to avoid the problem of trapped air creating bubbles and holes in the image. Today, Gates walks us through the slip-casting process for one of her jug forms using a four-piece plaster mold for the body and a two-piece plaster mold for the handle. Potter Linda Gates makes her work using multi-part ceramic molds, not only because it allows her to be creative with the forms, but also because it results in a smooth surface perfect for her digital decal decoration. From simple one-piece plaster molds to more complex multi-part molds, plaster mold making allows the potter the freedom to experiment with ideas for form and surface because it takes away a bit of the preciousness of a one-of-a-kind wheel thrown pot. While it was once thought of as an industrial process, using ceramic molds is a technique that is increasing in popularity among potters these days.
Kylian Mbappe, Juan Mata and host of football stars pay tribute to Eden Hazard, as he is hailed as 'one of the best ever' after announcing his retirement at 32.Mason Greenwood breaks his silence after scoring his first goal for Getafe - as on-loan Manchester United forward's post receives over one million likes on Instagram.
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