The changing patterns of antimicrobial resistance and shortage of novel classes of antibiotics have contributed to a demand for new antibacterial agents. coli remains a public health concern and is responsible for severe infections in humans. In addition, antibiotic resistance in foodborne E. The worldwide increase in the rate of resistance to carbapenem and colistin among members of the family Enterobacteriaceae, especially in developing countries, is a serious threat to their therapeutic use. STEC can be acquired by ingestion of contaminated food or water, or through contact with animals or their environment. It can also cause food-borne infections, such as those caused by enterohemorrhagic E. It can cause hospital- and community-acquired infections, resulting in diarrhea, meningitis, urinary tract infections (UTIs), bacteremia, pneumonia, surgical-site infections, and sepsis. coli is used as a contamination indicator for water, food, and agricultural products. It belongs to the family Enterobacteriaceae and as a pathogen causes serious urinary and gastrointestinal infections in humans. Annotation of the predicted genes revealed gene products belonging to several functional groups, including regulatory proteins, DNA packaging and phage structural proteins, host lysis proteins, and proteins involved in DNA/RNA metabolism and replication.Įscherichia coli is a Gram-negative, non-endospore-forming bacterium that is motile by means of peritrichous flagella. Comparison of predicted and experimental restriction digestion patterns allowed rough mapping of the physical ends of the phage genome, which was confirmed using the PhageTerm tool. The phage genome is 45,805 bp in length with a GC content of 50.3% and contains 76 predicted genes. The host range of the phage was found to be narrow, as it infected only two out of 137 clinical E. The diameter of the icosahedral head of fEg-Eco19 is 68 ± 2 nm, and the non-contractile tail length and diameter are 118 ± 0.2 and 13 ± 0.6 nm, respectively. Electron microscopy showed that the isolated phage is tailed and therefore belongs to the order Caudovirales, and morphologically, it resembles siphoviruses. Phage fEg-Eco19 formed clear, sharp-edged, round plaques. coli strain, was isolated from an Egyptian sewage sample. Phage fEg-Eco19, which is specific for a clinical E. ![]() The present study describes the isolation and characterization of a bacteriophage effective against the important clinical pathogen Escherichia coli, which shows increasing accumulation of antibiotic resistance. This, in turn, is important for the exploitation of phages for therapeutic purposes, as the use of uncharacterized phages may lead to treatment failure. Characterization of bacteriophages facilitates better understanding of their biology, host specificity, genomic diversity, and adaptation to their bacterial hosts.
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